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Proceedings Paper

Cell growth on surface-modified medical polymers
Author(s): Richard J. Sherlock; Daphne N. Bhogal; Michael Ball; Thomas J. Glynn
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Paper Abstract

Cellular reactions to implantable medical devices are dominated by the surface properties of materials from which the device is constructed. Consequently, in recent times much effort has been expended on modifying material surface properties to control bioactivity. We examine the effect of exposing surfaces to ultra-violet (UV) light from excimer lasers (λ = 193nm) in a room air environment. Working below the threshold of ablation, samples of nylon-12 and PET were treated. Physical and chemical studies of the surfaces following treatment demonstrated an increase in sample hydrophilicity, though no significant increases in roughness were recorded. Spectroscopic analyses revealed increased oxygen content in the surface layers while there were no chemical alterations in the bulk material. The assessment of in vitro interactions concerning the polymer samples and 3T3 fibroblast cells was conducted using cell counting, viability assays and a confocal microscopic analysis of cytoskeletal fluorescent staining. Results from cell counting and the viability tests confirmed that, subsequent to treatment, there was an increase in cell population on the surface, while improved spreading and activity was observed by confocal microscopy.

Paper Details

Date Published: 27 August 2003
PDF: 7 pages
Proc. SPIE 4876, Opto-Ireland 2002: Optics and Photonics Technologies and Applications, (27 August 2003); doi: 10.1117/12.463640
Show Author Affiliations
Richard J. Sherlock, National Ctr. for Laser Applications (Ireland)
Daphne N. Bhogal, National Ctr. for Laser Applications (Ireland)
Michael Ball, National Ctr. for Biomedical Engineering Science (Ireland)
Thomas J. Glynn, National Ctr. for Laser Applications (Ireland)

Published in SPIE Proceedings Vol. 4876:
Opto-Ireland 2002: Optics and Photonics Technologies and Applications
Vincent Toal; Norman Douglas McMillan; Gerard M. O'Connor; Eon O'Mongain; Austin F. Duke; John F. Donegan; James A. McLaughlin; Brian D. MacCraith; Werner J. Blau, Editor(s)

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