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Proceedings Paper

Optical and fluorescence properties of corn leaves from different nitrogen regimes
Author(s): Elizabeth M. Middleton; James E. McMurtrey; Petya K. Entcheva Campbell; Lawrence A. Corp; L. Maryn Butcher; Emmett W. Chappelle
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Paper Abstract

The important role of nitrogen (N) in limiting or enhancing vegetation productivity is relatively well understood, although the interaction of N with other environmental variables in natural and agricultural ecosystems needs more study. In 2001, a suite of optical, fluorescence, and biophysical measurements were collected on leaves of corn (Zea Mays L.) from field plots provided four N fertilizer application rates: 20%, 50%, 100% and 150% of optimal N levels. Two complementary sets of high-resolution (< 2 nm) optical spectra were acquired for both adaxial and abaxial leaf surfaces. The first was comprised of leaf optical properties (350-2500 nm) for reflectance, transmittance, and absorptance. The second was comprised of reflectance spectra (500-1000 nm) acquired with and without a long pass 665 nm filter to determine the fluorescence contribution to "apparent reflectance" in the 670-750 nm spectrum that includes the 685 and 740 nm chlorophyll fluorescence (ChlF) peaks. Two types of fluorescence measurements were also made on adaxial and abaxial surfaces: 1) fluorescence images in four 10 nm bands (blue, green, red, far-red) resulting from broadband irradiance excitation; and 2) emission spectra at 5 nm resolution produced by three excitation wavelengths (280, 380, and 532 nm). The strongest relationships between optical properties and foliar chemistry were obtained for a "red-edge" optical parameter versus C/N and chlorophyll b. Select optical indices and ChlF parameters were correlated. A significant contribution of steady-state ChlF to apparent reflectance was observed, averaging 10-25% at 685 nm and 2-6% at 740 nm over the range of N treatments. From all measurements assessing fluorescence, higher ChlF was measured from the abaxial leaf surfaces.

Paper Details

Date Published: 17 March 2003
PDF: 12 pages
Proc. SPIE 4879, Remote Sensing for Agriculture, Ecosystems, and Hydrology IV, (17 March 2003); doi: 10.1117/12.463087
Show Author Affiliations
Elizabeth M. Middleton, NASA Goddard Space Flight Ctr. (United States)
James E. McMurtrey, USDA/Agricultural Research Service (United States)
Petya K. Entcheva Campbell, NASA Goddard Space Flight Ctr. (United States)
Lawrence A. Corp, NASA Goddard Space Flight Ctr. and USDA/Agricultural Research Service (United States)
L. Maryn Butcher, NASA Goddard Space Flight Ctr. and USDA/Agricultural Research Service (United States)
Emmett W. Chappelle, NASA Goddard Space Flight Ctr. and USDA/Agricultural Research Service (United States)


Published in SPIE Proceedings Vol. 4879:
Remote Sensing for Agriculture, Ecosystems, and Hydrology IV
Manfred Owe; Guido D'Urso; Leonidas Toulios, Editor(s)

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