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Proceedings Paper

Imaging of green fluorescent protein in live plant by scanning near-field optical microscopy
Author(s): Jianhua Xu; Tao Chen; Jialin Sun; Jihua Guo; Jun Zhao
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Paper Abstract

An auxin/IAA induced in vivo green fluorescent protein (GFP) in a living plant Arabidopsis root has been studied by a scanning near-field microscope in transmission mode. The promising near-field images of the inducible GFPs at sub- surface of a plant cell suggest that they may locate proximity to the cell wall, i.e. both sides of and in the cytoplasm membrane. The clear and faint fluorescent spots with 1-3 micrometers showed that the proteins localized nearer and farther to the cell wall, respectively. All GFP molecules gathered together in a cell, and no individual GFP was observed in the experiment.

Paper Details

Date Published: 12 April 2002
PDF: 5 pages
Proc. SPIE 4536, International Workshop on Photonics and Imaging in Biology and Medicine, (12 April 2002); doi: 10.1117/12.462571
Show Author Affiliations
Jianhua Xu, Tsinghua Univ. (China)
Tao Chen, Tsinghua Univ. (China)
Jialin Sun, Tsinghua Univ. (China)
Jihua Guo, Tsinghua Univ. (China)
Jun Zhao, Tsinghua Univ. (China)

Published in SPIE Proceedings Vol. 4536:
International Workshop on Photonics and Imaging in Biology and Medicine
Qingming Luo; Britton Chance; Valery V. Tuchin, Editor(s)

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