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Proceedings Paper

Phosphorescence lifetime measurement using confocal microscopy
Author(s): Chengjun Li; Shaoqun Zeng; Qingming Luo; Wei Zhou; Guoqing Zhang
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Paper Abstract

The continuous light coming from the Argon Gas Ion Laser can be converted to pulse light when an optical chopper is equipped. The sample is excited by the pulse light in the confocal microscopy system. The light intensity of the excitation light and the phosphorescence is simultaneously recorded in terms of gray value by the confocal microscopy system. The phosphorescence lifetime measurement can be realized with time-resolved analysis for the phosphorescence intensity decay. The different decay lifetimes of the Oxy- Phor R2 sample under different oxygen concentration are measured with the reconstructed confocal microscopy system.

Paper Details

Date Published: 12 April 2002
PDF: 5 pages
Proc. SPIE 4536, International Workshop on Photonics and Imaging in Biology and Medicine, (12 April 2002); doi: 10.1117/12.462566
Show Author Affiliations
Chengjun Li, Huazhong Univ. of Science and Technology (China)
Shaoqun Zeng, Huazhong Univ. of Science and Technology (China)
Qingming Luo, Huazhong Univ. of Science and Technology (China)
Wei Zhou, Huazhong Univ. of Science and Technology (China)
Guoqing Zhang, Huazhong Univ. of Science and Technology (China)


Published in SPIE Proceedings Vol. 4536:
International Workshop on Photonics and Imaging in Biology and Medicine

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