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Proceedings Paper

5-HT spatial distribution imaging with multiphoton excitation of 5-HT correlative visible fluorescence in live cells
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Paper Abstract

The autofluorescence of 5-Hydroxytryptamine (5-HT) loaded rat mucosal mast cells (RBL-2H3 cells) is imaged with multiphoton excitation laser scanning microscope (MPELSM). 5-HT correlative visible fluorescence (Fco-vis) excited with 740-nm multiphoton excitation is observed in live cells for the first time, and the generating mechanism of 5-HT Fco-vis is studied. The spatial distribution of 5-HT in live cells is imaged at high spatial resolution in our experiment, which provides a new way to study the correlation between 5-HT spatial distribution and content, and the cellular functional state in live tissue or cells.

Paper Details

Date Published: 12 April 2002
PDF: 7 pages
Proc. SPIE 4536, International Workshop on Photonics and Imaging in Biology and Medicine, (12 April 2002); doi: 10.1117/12.462565
Show Author Affiliations
Zhihong Zhang, Huazhong Univ. of Science and Technology (China)
Shaoqun Zeng, Huazhong Univ. of Science and Technology (China)
Yafeng Liu, Huazhong Univ. of Science and Technology (China)
Wei Zhou, Huazhong Univ. of Science and Technology (China)
Tongsheng Chen, Huazhong Univ. of Science and Technology (China)
Qingming Luo, Huazhong Univ. of Science and Technology (China)


Published in SPIE Proceedings Vol. 4536:
International Workshop on Photonics and Imaging in Biology and Medicine

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