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Proceedings Paper

High-sensitivity detection of capillary electrophoresis with multiphoton excitation
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Paper Abstract

Multiphoton excited fluorescence detection is a powerful tool for probing chemistry and structure deep within biological tissue, for performing sensitive measurement on deep-UV (ex 200-300 nm) specimen. We present an approach for rapid analysis of Rhodamine B using multiphoton excited fluorescence detection coupled to capillary electrophoresis separations. In this highly versatile approach, Rhodamine B is excited through the nearly simultaneous absorption of two low-energy photons, fluorescence can be detected efficiently with low background. In these studies, Rhodamine B is fractionated in several minutes, with mass detection limits as low as 10 amol (2nM). This approach is demonstrated to be a powerful tool for analyzing the complex biological samples of minute quantities.

Paper Details

Date Published: 12 April 2002
PDF: 7 pages
Proc. SPIE 4536, International Workshop on Photonics and Imaging in Biology and Medicine, (12 April 2002); doi: 10.1117/12.462561
Show Author Affiliations
Ling Fu, Huazhong Univ. of Science and Technology (China)
Qiang Lu, Huazhong Univ. of Science and Technology (China)
Hong Chen, Wuhan Univ. (China)
Tongsheng Chen, Huazhong Univ. of Science and Technology (China)
Shaoqun Zeng, Huazhong Univ. of Science and Technology (China)
Qingming Luo, Huazhong Univ. of Science and Technology (China)


Published in SPIE Proceedings Vol. 4536:
International Workshop on Photonics and Imaging in Biology and Medicine

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