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Proceedings Paper

Electrochemiluminescence immunoassay of P53 from human SWO-38 glioma cell
Author(s): Guihong Yan; Yonghong He; Da Xing; Yadong Wei; Shici Tan
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Paper Abstract

Electrochemiluminescence (ECL) technique for bimolecular detection offers ultra-high sensitivity and good signal-to- noise ratio. In this work, an ECL reaction and detection system were established. We used a big working electrode and a single photon counter to heighten the detection limit. The results showed that the detection limit of the system is 1/1000 fmol/L free labeled Ru(bpy)3 2+. The concentration dependence of ECL intensity was not linear in the range of low concentration (fmol/L level) free labeled Ru(bpy)3 2+. While in pmol/L level, the ECL intensity was linear. Ru(bpy)3 2+ + TPA (tripropylmine) ECL reaction system was used to indicate P53 protein from human SWO-38 cell line, which was established from grade 1-11 glioma tumor. This method could have potential applications in early-stage tumor diagnosis and environmental inspection.

Paper Details

Date Published: 12 April 2002
PDF: 7 pages
Proc. SPIE 4536, International Workshop on Photonics and Imaging in Biology and Medicine, (12 April 2002); doi: 10.1117/12.462550
Show Author Affiliations
Guihong Yan, South China Normal Univ. (China)
Yonghong He, South China Normal Univ. (China)
Da Xing, South China Normal Univ. (China)
Yadong Wei, South China Normal Univ. (China)
Shici Tan, South China Normal Univ. (China)


Published in SPIE Proceedings Vol. 4536:
International Workshop on Photonics and Imaging in Biology and Medicine

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