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Proceedings Paper

Investigation of singlet oxygen generation in Vit C-Cu2+-LDL system by chemiluminescence method
Author(s): Juan Wang; Da Xing; Shici Tan; Yonghong Tang; Yonghong He
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Paper Abstract

In this study, by chemiluminescence method using a Cypridina luciferin analog, 2-methyl-6-(p-methoxyphenyl)-3,7- dihydroimidazo[1,2-a]pyrazin-3-one (MCLA), as a selective and sensitive chemiluminescence probe, singlet oxygen (1O2) formation was observed in the vit C- LDL-Cu2+ reaction system. Another experimental evidence for the generation of 1O2 was the quenching effect of sodium azide (NaN3) on vit C-induced chemiluminescence in the reaction mixture of LDL- Cu2+-MCLA. Analysis based on the experimental results indicated the plausible reaction mechanism is that vit C converts Cu2+ to its reduced state and vit C becomes vit C radical itself, thereby stimulating the formation of peroxyl radicals, and bimolecular reaction of peroxyl radicals results in 1O2 production in the above systems.

Paper Details

Date Published: 12 April 2002
PDF: 7 pages
Proc. SPIE 4536, International Workshop on Photonics and Imaging in Biology and Medicine, (12 April 2002); doi: 10.1117/12.462544
Show Author Affiliations
Juan Wang, South China Normal Univ. (China)
Da Xing, South China Normal Univ. (China)
Shici Tan, South China Normal Univ. (China)
Yonghong Tang, South China Normal Univ. (China)
Yonghong He, South China Normal Univ. (China)


Published in SPIE Proceedings Vol. 4536:
International Workshop on Photonics and Imaging in Biology and Medicine

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