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Proceedings Paper

Investigation of phosphatidylcholine enhancing FITC-insulin across buccal mucosa by confocal laser scanning microscopy
Author(s): Weiqun Tian; Li Su; Shaoqun Zeng; Qingming Luo; Qiuhua Gao; Huibi Xu
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Paper Abstract

The aim was to characterize the transport of fluorescein isothiocyanate (FITC)-labeled dextran and insulin with different resoluble compounds for peptides and proteins through buccal mucosa. The penetration rate of insulin molecules through porcine buccal mucosa (a nonkeratinized epithelium, comparable to human buccal mucosa) was investigated by measuring transbuccal fluxes and by analyzing the distribution of the fluorescent probe in the rabbit buccal mucosa epithelium, using confocal laser scanning microscopy for visualizing permeation pathways. The confocal images of the distribution pattern of FITC-dextran and FITC-insulin showed that the paracellular route is the major pathway of FITC-dextran through buccal mucosa epithelium, the intra-cellular route is the major pathway of FITC-insulin through buccal mucosa epithelium. The permeation rate can be increased by co-administration of soybean phosphatidylcholine (SPC).

Paper Details

Date Published: 12 April 2002
PDF: 6 pages
Proc. SPIE 4536, International Workshop on Photonics and Imaging in Biology and Medicine, (12 April 2002); doi: 10.1117/12.462526
Show Author Affiliations
Weiqun Tian, Huazhong Univ. of Science and Technology (China)
Li Su, Huazhong Univ. of Science and Technology (China)
Shaoqun Zeng, Huazhong Univ. of Science and Technology (China)
Qingming Luo, Huazhong Univ. of Science and Technology (China)
Qiuhua Gao, Huazhong Univ. of Science and Technology (China)
Huibi Xu, Huazhong Univ. of Science and Technology (China)


Published in SPIE Proceedings Vol. 4536:
International Workshop on Photonics and Imaging in Biology and Medicine

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