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Proceedings Paper

Early mineralization of normal and pathologic calvaria as revealed by Raman spectroscopy
Author(s): Michael D. Morris; Shona Stewart; Catherine Perso Tarnowski; Dana Shea; Renny Franceschi; Dian Wang; Michael A. Ignelzi; Wei Wang; Evan T. Keller; Din-Lii Lin; Steven A. Goldstein; Juan M. Taboas
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Paper Abstract

Bone tissue consists of a carbonated apatite-like mineral supported on a hydrated, collagen-rich protein matrix. Despite extensive studies into the macroscopic characteristics of bone, much about the early stages of bone formation remains unknown. Raman microspectroscopy and imaging are increasingly important tools for the study of mineralized tissue, due to advancements in both spectral acquisition and analysis protocols. With this technique, mapping of both organic and inorganic components of bone, in addition to determining their distributions with high spatial resolution across a specimen, can be realized. We have employed Raman microscopy to investigate the early stages of mineralization in four different mouse calvarial systems: typical and atypical osteoblastic (bone forming) cell cultures and healthy and diseased bone tissue. These systems are commonly utilized as models for mineralization. The mineral deposited by osteoblast cultures grown atypically gives a Raman signal completely different to that observed in osteoblast cultures grown in the conventional manner. Similarly, Raman images of healthy and diseased bone tissue show differences in the relationship of the mineral and matrix environments. In this report, we compare the several differences between these four mineral environments, and discuss the chemistry of mineral maturation observed.

Paper Details

Date Published: 27 March 2002
PDF: 12 pages
Proc. SPIE 4614, Biomedical Vibrational Spectroscopy II, (27 March 2002); doi: 10.1117/12.460796
Show Author Affiliations
Michael D. Morris, Univ. of Michigan (United States)
Shona Stewart, Univ. of Michigan (United States)
Catherine Perso Tarnowski, Univ. of Michigan (United States)
Dana Shea, Univ. of Michigan (United States)
Renny Franceschi, Univ. of Michigan (United States)
Dian Wang, Univ. of Michigan (United States)
Michael A. Ignelzi, Univ. of Michigan (United States)
Wei Wang, Univ. of Michigan (United States)
Evan T. Keller, Univ. of Michigan (United States)
Din-Lii Lin, Univ. of Michigan (United States)
Steven A. Goldstein, Univ. of Michigan (United States)
Juan M. Taboas, Univ. of Michigan (United States)

Published in SPIE Proceedings Vol. 4614:
Biomedical Vibrational Spectroscopy II
Anita Mahadevan-Jansen; Henry H. Mantsch; Gerwin J. Puppels, Editor(s)

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