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Proceedings Paper

Raman imaging and spectroscopy of single functional erythrocytes: a feasibility study
Author(s): Kerstin Ramser; Erik J. Bjerneld; Camilla Fant; Mikael Kall
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Paper Abstract

Hemoglobin (Hb) within single erythrocytes (red blood cells), adsorbed on poly-lysine coated glass surfaces, was studied using resonance Raman spectroscopy and global Raman imaging. The erythrocytes were found to be sensitive to both surface adsorption and to the laser light. Topological changes of the cell membrane were observed immediately after cell adsorption in Raman images. We observed a photo-induced increase of the fluorescence background occurring simultaneously with a decrease in the Hb Raman signal. Concurrent changes in Raman spectra revealed a conversion of oxy-Hb to the met-Hb state. However, at a low accumulated photon dose, the preparation method enabled the recording of Raman spectra during the oxygenation cycle of a single red blood cell in buffer, which shows that Hb was in an in-vivo environment. Thus, Raman spectroscopy of functional Hb in isolated red blood cells is feasible.

Paper Details

Date Published: 27 March 2002
PDF: 8 pages
Proc. SPIE 4614, Biomedical Vibrational Spectroscopy II, (27 March 2002); doi: 10.1117/12.460794
Show Author Affiliations
Kerstin Ramser, Chalmers Univ. of Technology (Sweden)
Erik J. Bjerneld, Chalmers Univ. of Technology (Sweden)
Camilla Fant, Goeteborg Univ. (Sweden)
Mikael Kall, Chalmers Univ. of Technology (Sweden)


Published in SPIE Proceedings Vol. 4614:
Biomedical Vibrational Spectroscopy II
Anita Mahadevan-Jansen; Henry H. Mantsch; Gerwin J. Puppels, Editor(s)

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