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Proceedings Paper

Skin hydration by spectroscopic imaging using multiple near-infrared bands
Author(s): E. Michael Attas; Michael G. Sowa; Trevor B. Posthumus; Bernhard J. Schattka; Henry H. Mantsch; Shuliang L. Zhang
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Paper Abstract

Near-infrared spectroscopic methods have been developed to determine the degree of hydration of human skin in vivo. Reflectance spectroscopic imaging was used to investigate the distribution of skin moisture as a function of location. A human study in a clinical setting has generated quantitative data showing the effects of a drying agent and a moisturizer on delineated regions of the forearms of eight volunteers. Two digital imaging systems equipped with liquid-crystal tunable filters were used to collect stacks of monochromatic images at 10-nm intervals over the wavelength bands 650-1050 nm and 960-1700 nm. Images generated from measurements of water absorption-band areas at three different near-IR wavelengths (970, 1200, and 1450 nm) showed obvious differences in the apparent distribution of water in skin. Changes resulting from the skin treatments were much more evident in the 1200-nm and 1450-nm images than in the 970-nm ones. The variable sensitivity of the method at different wavelengths has been interpreted as being the result of different penetration depths of the infrared light used in the reflectance studies. Ex-vivo experiments with pigskin have provided evidence supporting the relationship between wavelength and penetration depth. Combining the hydration results from several near-IR water bands allows additional information on hydration depth to be obtained.

Paper Details

Date Published: 27 March 2002
PDF: 10 pages
Proc. SPIE 4614, Biomedical Vibrational Spectroscopy II, (27 March 2002); doi: 10.1117/12.460783
Show Author Affiliations
E. Michael Attas, National Research Council Canada (Canada)
Michael G. Sowa, National Research Council Canada (Germany)
Trevor B. Posthumus, National Research Council Canada (Canada)
Bernhard J. Schattka, National Research Council Canada (Canada)
Henry H. Mantsch, National Research Council Canada (Canada)
Shuliang L. Zhang, Unilever Research (United States)

Published in SPIE Proceedings Vol. 4614:
Biomedical Vibrational Spectroscopy II
Anita Mahadevan-Jansen; Henry H. Mantsch; Gerwin J. Puppels, Editor(s)

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