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Proceedings Paper

Application of luminescent CdSe-ZnS quantum dot bioconjugates in immuno- and fluorescence-quenching assays
Author(s): Phan T. Tran; Ellen R. Goldman; George P. Anderson; J. Matthew Mauro; Hedi M. Mattoussi
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Paper Abstract

Colloidal CdSe-ZnS semiconductor quantum dots (QDs) have tunable narrow-band luminescence emission and high resistance to photodegradation, properties that make them promising for use as fluorescent labels in biotechnological applications such as fluoro-immunoassays and in cellular imaging. We have developed efficient methods for conjugating these inorganic fluorophores to antibodies via the use of a molecular adaptor protein, the B2 domain of streptococcal protein G appended with an electrostatic interaction domain, which acts as a bridge between the QD fluorophore and antibodies. In this approach, coupling of the adaptor to the QD is driven by electrostatic self-assembly, while bridging to the antibody is driven by specific interactions. In the present study, we present fluoro-immunoassay studies employing QD-antibody conjugates for the detection of low levels of the explosive hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX). We will also explore the use of self-assembled QD-protein-receptor complexes in Foerster energy transfer assays, where the QD emission can be altered by a quenching dye receptor in a QD-protein-receptor conjugate.

Paper Details

Date Published: 18 March 2002
PDF: 8 pages
Proc. SPIE 4636, Nanoscience Using Laser-Solid Interactions, (18 March 2002); doi: 10.1117/12.459733
Show Author Affiliations
Phan T. Tran, Naval Research Lab. (United States)
Ellen R. Goldman, Naval Research Lab. (United States)
George P. Anderson, Naval Research Lab. (United States)
J. Matthew Mauro, Naval Research Lab. (United States)
Hedi M. Mattoussi, Naval Research Lab. (United States)

Published in SPIE Proceedings Vol. 4636:
Nanoscience Using Laser-Solid Interactions
Kouichi Murakami; David B. Geohegan; Frank Traeger, Editor(s)

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