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Proceedings Paper

Rapid detection of bacteria with miniaturized pyrolysis-gas chromatographic analysis
Author(s): Curtis Mowry; Catherine H. Morgan; Quentin Baca; Ronald P. Manginell; Richard J. Kottenstette; Patrick Lewis; Gregory C. Frye-Mason
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Paper Abstract

Rapid detection and identification of bacteria and other pathogens is important for many civilian and military applications. The profiles of biological markers such as fatty acids can be used to characterize biological samples or to distinguish bacteria at the gram-type, genera, and even species level. Common methods for whole cell bacterial analysis are neither portable nor rapid, requiring lengthy, labor intensive sample preparation and bench-scale instrumentation. These methods chemically derivatize fatty acids to produce more volatile fatty acid methyl esters (FAMEs) that can be separated and analyzed by a gas chromatograph (GC)/mass spectrometer. More recent publications demonstrate decreased sample preparation time with in situ derivatization of whole bacterial samples using pyrolysis/derivatization. Ongoing development of miniaturized pyrolysis/GC instrumentation by this department capitalizes on Sandia advances in the field of microfabricated chemical analysis systems ((mu) ChemLab). Microdevices include rapidly heated stages capable of pyrolysis or sample concentration, gas chromatography columns, and surface acoustic wave (SAW) sensor arrays. We will present results demonstrating the capabilities of these devices toward fulfilling the goal of portable, rapid detection and early warning of the presence of pathogens in air or water.

Paper Details

Date Published: 21 February 2002
PDF: 8 pages
Proc. SPIE 4575, Chemical and Biological Early Warning Monitoring for Water, Food, and Ground, (21 February 2002); doi: 10.1117/12.456911
Show Author Affiliations
Curtis Mowry, Sandia National Labs. (United States)
Catherine H. Morgan, Sandia National Labs. (United States)
Quentin Baca, Sandia National Labs. (United States)
Ronald P. Manginell, Sandia National Labs. (United States)
Richard J. Kottenstette, Sandia National Labs. (United States)
Patrick Lewis, Sandia National Labs. (United States)
Gregory C. Frye-Mason, Sandia National Labs. (United States)


Published in SPIE Proceedings Vol. 4575:
Chemical and Biological Early Warning Monitoring for Water, Food, and Ground
Janet L. Jensen; Larry W. Burggraf, Editor(s)

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