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Proceedings Paper

Concentration measurement of lysosome enzymes in blood by fluorimetric analysis method
Author(s): Marina M. Strinadko; Elena M. Strinadko
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Paper Abstract

The diagnostics of heritable disease series and sugar diabetes, myocardial infarction, collagenosis and kidney diseases widely uses the measurement of lysosomic enzymes in blood. In the present research work the definition procedure of concentration (beta) -glucuronidase with the help of fluorimetric analysis is offered, which allows using microamounts of biological fluids and samples with low enzyme activity which is especially important in paediatric practice. Due to the sharp sensibility of fluorimetric analysis and high speed of luminescent reactions the procedure gives an opportunity to obtain the result in the minimum terms as well as the use of small amounts of reaction mixture. The incubation in large dilution leads thereby to the elimination of influence of endogenic inhibitors and activators.

Paper Details

Date Published: 5 February 2002
PDF: 4 pages
Proc. SPIE 4607, Selected Papers from Fifth International Conference on Correlation Optics, (5 February 2002); doi: 10.1117/12.455227
Show Author Affiliations
Marina M. Strinadko, Bukovinian State Medical Academy (Ukraine)
Elena M. Strinadko, Bukovinian State Medical Academy (Ukraine)


Published in SPIE Proceedings Vol. 4607:
Selected Papers from Fifth International Conference on Correlation Optics

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