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Proceedings Paper

Computer-controlled laser ablation: a novel tool for biomolecular patterning
Author(s): Jonathan P. Wright; Chitladda Mahanivong; Duy K. Pham; Dan V. Nicolau; K. Suyama; Masamitsu Shirai; Masahiro Tsunooka
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Paper Abstract

This paper describes a novel laser-based method for preparing microchannels in a bilayer system consisting of a UV sensitive polymer, acetophenone O-acryloyloxime (AAPO), layered with bovine serum albumin (BSA); BSA acts as a common blocking agent to prevent biomolecular attachment to the unexposed regions. The focus of the paper is on the use of a computer-controlled laser ablation system comprising a research-grade inverted optical microscope, a pulsed nitrogen laser emitting at 337 nm and a programmable X-Y-Z stage. By using a 100x oil immersion objective, channels of 1micrometers width and ca. 1 mm depth can be etched into the BSA-coated polymer. The precise width of the channel can be controlled by simply adjusting both the laser power and focusing. The addition of myosin to the base of these channels provides tracks on which actin filaments can move. By adjusting the width of the tracks, it is possible to regulate the direction of motion of the actin filaments.

Paper Details

Date Published: 19 November 2001
PDF: 9 pages
Proc. SPIE 4590, BioMEMS and Smart Nanostructures, (19 November 2001); doi: 10.1117/12.454620
Show Author Affiliations
Jonathan P. Wright, Swinburne Univ. of Technology (Australia)
Chitladda Mahanivong, Swinburne Univ. of Technology (Australia)
Duy K. Pham, Swinburne Univ. of Technology (Australia)
Dan V. Nicolau, Swinburne Univ. of Technology (Australia)
K. Suyama, Osaka Prefecture Univ. (Japan)
Masamitsu Shirai, Osaka Prefecture Univ. (Japan)
Masahiro Tsunooka, Osaka Prefecture Univ. (Japan)

Published in SPIE Proceedings Vol. 4590:
BioMEMS and Smart Nanostructures
Laszlo B. Kish; Erol C. Harvey; William B. Spillman, Editor(s)

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