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Proceedings Paper

Large-aperture CCD x-ray detector for protein crystallography using a fiber-optic taper
Author(s): Michael G. Strauss; Edwin M. Westbrook; Istvan Naday; Tom A. Coleman; Mary L. Westbrook; Dale J. Travis; Robert M. Sweet; J. W. Pflugrath; Martin J. Stanton
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Paper Abstract

A detector with a 114 mm aperture, based on a charge-coupled device (CCD), has been designed for x-ray diffraction studies in protein crystallography. The detector was tested on a beamline of the National Synchrotron Light Source at Brookhaven National Laboratory with a beam intensity greater than 109 x-ray photons/s. A fiber-optic taper, an image intensifier, and a lens demagnify, intensify, and focus the image onto a CCD having 512 X 512 pixels. A detective quantum efficiency (DQE) of 0.36 was obtained by evaluating the statistical uncertainty in the detector output. The dynamic range of a 4 X 4 pixel resolution element, comparable in size to a diffraction peak, was 10 4. The point-spread function shows FWHM resolution of approximately 1 pixel, where a pixel on the detector face is 160 micrometers . A complete data set, consisting of forty-five 1 degree(s) rotation frames, was obtained in just 36 s of x-ray exposure to a crystal of chicken egg-white lysozyme. In a separate experiment, a lysozyme data set consisting of 495 0.1 degree(s) frames, was processed by the MADNES data reduction program, yielding symmetry R-factors for the data of 3.2- 3.5. Diffraction images from crystals of the myosin S1 head (a equals 275 angstroms) were also recorded. The Bragg spots, only 5 pixels apart, were resolved but were not sufficiently separated to process these data. Changes in the detector design which will improve the DQE and spatial resolution are outlined. The overall performance showed that this type of detector is well suited for x-ray scattering investigations with synchrotron sources.

Paper Details

Date Published: 1 July 1991
PDF: 16 pages
Proc. SPIE 1447, Charge-Coupled Devices and Solid State Optical Sensors II, (1 July 1991); doi: 10.1117/12.45312
Show Author Affiliations
Michael G. Strauss, Argonne National Lab. (United States)
Edwin M. Westbrook, Argonne National Lab. (United States)
Istvan Naday, Argonne National Lab. (United States)
Tom A. Coleman, Argonne National Lab. (United States)
Mary L. Westbrook, Argonne National Lab. (United States)
Dale J. Travis, Argonne National Lab. (United States)
Robert M. Sweet, Brookhaven National Lab. (United States)
J. W. Pflugrath, Cold Spring Harbor Lab. (United States)
Martin J. Stanton, Brandeis Univ. (United States)

Published in SPIE Proceedings Vol. 1447:
Charge-Coupled Devices and Solid State Optical Sensors II
Morley M. Blouke, Editor(s)

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