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Proceedings Paper

High-resolution element mapping inside biological samples using fluorescence microtomography
Author(s): Christian G. Schroer; Boris Benner; Til Florian Guenzler; Marion Kuhlmann; Bruno Lengeler; Walter H. Schroeder; Arnd J. Kuhn; Alexandre S. Simionovici; Anatoly A. Snigirev; Irina Snigireva
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Paper Abstract

Sample preparation for element analysis of many biological tissues is difficult to achieve and prone to introduce contamination. Using x-ray fluorescence element microtomography (XFEMT) the element distribution on a virtual section across the sample can be determined with a resolution in the micrometer range. Fluorescence microtomograms of two plant samples are shown, demonstrating the possibility to map physiologically relevant ions, trace elements, and heavy metal pollutants at the cellular level. Attenuation effects inside the plant are corrected by a self-consistent tomographic reconstruction technique.

Paper Details

Date Published: 7 January 2002
PDF: 10 pages
Proc. SPIE 4503, Developments in X-Ray Tomography III, (7 January 2002); doi: 10.1117/12.452850
Show Author Affiliations
Christian G. Schroer, RWTH-Aachen (Germany)
Boris Benner, RWTH-Aachen (Germany)
Til Florian Guenzler, RWTH-Aachen (Germany)
Marion Kuhlmann, RWTH-Aachen (Germany)
Bruno Lengeler, RWTH-Aachen (Germany)
Walter H. Schroeder, Forschungszentrum Juelich GmbH (Germany)
Arnd J. Kuhn, Forschungszentrum Juelich GmbH (Germany)
Alexandre S. Simionovici, European Synchrotron Radiation Facility (France)
Anatoly A. Snigirev, European Synchrotron Radiation Facility (France)
Irina Snigireva, European Synchrotron Radiation Facility (France)


Published in SPIE Proceedings Vol. 4503:
Developments in X-Ray Tomography III
Ulrich Bonse, Editor(s)

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