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Proceedings Paper

Segmentation, autofocusing, and signature extraction of tuberculosis sputum images
Author(s): Manuel G. Forero-Vargas; Filip Sroubek; Josue Alvarez-Borrego; Norberto Malpica; Gabriel Cristobal; Andres Santos; Luis Alcala; Manuel Desco; Leon Cohen
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Paper Abstract

Bacteria segmentation of particular species entails a challenging process. Bacteria shape is not enough as a discriminant feature, because there are many species that share the same shape. We present here two methods for tuberculosis image segmentation using the chromatic information. The first method is based on fuzzy segmentation of the color images based on the information that it is entailed in each separate chromatic histogram. The second method is a simple color filtering account by comparison of the inverse of the yellowish stained bacteria (blue channel) with the product of the other two chromatic channels. The third method is based on the extraction of image signatures by projecting logarithmic-polar mappings onto 1D vectors. This representation provides a very compact description of all image aspects, including shape, texture and color. An achromatic segmentation method is also presented based on the use of gray-level morphological operators only to the green channel. Finally we present the results of different autofocusing algorithms of stained tuberculosis images.

Paper Details

Date Published: 15 November 2002
PDF: 12 pages
Proc. SPIE 4788, Photonic Devices and Algorithms for Computing IV, (15 November 2002); doi: 10.1117/12.451665
Show Author Affiliations
Manuel G. Forero-Vargas, Univ. Nacional de Colombia (Colombia)
Filip Sroubek, Institute of Information Theory and Automation (Czech Republic)
Josue Alvarez-Borrego, Centro de Investigacion Cientifica y de Educacion Superior de Ensenada (Mexico)
Norberto Malpica, Cidudad Univ. (Spain)
Gabriel Cristobal, Instituto de Optica/CSIC (Spain)
Andres Santos, Ciudad Univ. (Spain)
Luis Alcala, Hospital General Universitario Gregorio Marañón (Spain)
Manuel Desco, Hospital General Universitario Gregorio Marañón (Spain)
Leon Cohen, CUNY/Hunter College (United States)

Published in SPIE Proceedings Vol. 4788:
Photonic Devices and Algorithms for Computing IV
Khan M. Iftekharuddin; Abdul Ahad S. Awwal, Editor(s)

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