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Proceedings Paper

Nanostructures of organic molecules and proteins on surfaces
Author(s): Nabil A. Amro; Jayne C. Garno; Maozi Liu; Kapila Wadu-Mesthrige; Gang-yu Liu
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Paper Abstract

Patterning bioreceptors on surfaces is a key step in the fabrication of biosensors and biochips. State-of-the art technology can produce micrometer-sized biostructures, however, further miniaturization at the nanoscale will require new methods and lithographic tools. In this proceeding, we report three approaches: nanopen reader and writer (NPRW), nanografting and latex particle lithography; for creating nanostructures of small molecules, DNA and proteins. Using nanografting and NPRW, nanostructures of thiol molecules or thiolated ssDNA are fabricated within self-assembled monolayers. Proteins attach selectively to nanopatterns of thiol molecules containing bioadhesive groups such as aldehyde or carboxylates. Using latex particle lithography, arrays of protein nanostructures are produced with high throughput on mica and gold substrates. Near-physiological conditions are used in structural characterization, thus the orientation, reactivity and stability of proteins and DNA molecules within nanostructures may be monitored directly via AFM. While AFM-based approaches provide the highest precision, nanoparticle lithography can produce arrays of protein nanostructures with high throughput. The nanostructures of proteins produced by these approaches provide an excellent opportunity for fundamental investigations of biochemical reactions on surfaces, such as antigen-antibody recognition and DNA-protein interactions. These methods provide a foundation for advancing biotechnology towards the nanoscale.

Paper Details

Date Published: 15 November 2002
PDF: 13 pages
Proc. SPIE 4807, Physical Chemistry of Interfaces and Nanomaterials, (15 November 2002); doi: 10.1117/12.451241
Show Author Affiliations
Nabil A. Amro, Univ. of California/Davis (United States)
Jayne C. Garno, Univ. of California/Davis and Wayne State Univ. (United States)
Maozi Liu, Univ. of California/Davis (United States)
Kapila Wadu-Mesthrige, Wayne State Univ. (United States)
Gang-yu Liu, Univ. of California/Davis (United States)

Published in SPIE Proceedings Vol. 4807:
Physical Chemistry of Interfaces and Nanomaterials
Jin Z. Zhang; Zhong L. Wang, Editor(s)

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