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Proceedings Paper

Surface plasmon resonance imaging system with Mach-Zehnder phase-shift interferometry for DNA micro-array hybridization
Author(s): Feng-Ming Hsiu; Shean-Jen Chen; Chien-Hung Tsai; Chia-Yuan Tsou; Y.-D. Su; G.-Y. Lin; K.-T. Huang; Jin-Jung Chyou; Wei-Chih Ku; S.-K. Chiu; C.-M. Tzeng
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Paper Abstract

Surface plasmon resonance (SPR) imaging system is presented as a novel technique based on modified Mach-Zehnder phase-shifting interferometry (PSI) for biomolecular interaction analysis (BIA), which measures the spatial phase variation of a resonantly reflected light in biomolecular interaction. In this technique, the micro-array SPR biosensors with over a thousand probe NDA spots can be detected simultaneously. Owing to the feasible and swift measurements, the micro-array SPR biosensors can be extensively applied to the nonspecific adsorption of protein, the membrane/protein interactions, and DNA hybridization. The detection sensitivity of the SPR PSI imaging system is improved to about 1 pg/mm2 for each spot over the conventional SPR imaging systems. The SPR PSI imaging system and its SPR sensors have been successfully used to observe slightly index change in consequence of argon gas flow through the nitrogen in real time, with high sensitivity, and at high-throughout screening rates.

Paper Details

Date Published: 25 September 2002
PDF: 8 pages
Proc. SPIE 4819, Polarization Measurement, Analysis, and Applications V, (25 September 2002); doi: 10.1117/12.450922
Show Author Affiliations
Feng-Ming Hsiu, National Central Univ. (Taiwan)
Shean-Jen Chen, National Central Univ. (Taiwan)
Chien-Hung Tsai, National Central Univ. (Taiwan)
Chia-Yuan Tsou, National Central Univ. (Taiwan)
Y.-D. Su, National Central Univ. (Taiwan)
G.-Y. Lin, National Central Univ. (Taiwan)
K.-T. Huang, National Central Univ. (Taiwan)
Jin-Jung Chyou, National Central Univ. (Taiwan)
Wei-Chih Ku, U-Vision Biotech Inc. (Taiwan)
S.-K. Chiu, U-Vision Biotech Inc. (Taiwan)
C.-M. Tzeng, U-Vision Biotech Inc. (Taiwan)

Published in SPIE Proceedings Vol. 4819:
Polarization Measurement, Analysis, and Applications V
Dennis H. Goldstein; David B. Chenault, Editor(s)

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