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Proceedings Paper

Application of FTIR microspectroscopy for the follow-up of childhood leukemia chemotherapy
Author(s): Shaul Mordechai; J. Mordehai; Jagannathan Ramesh; C. Levi; Mahmud Huleihal; Vitaly Erukhimovitch; A. Moser; J. Kapelushnik
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Paper Abstract

Acute Lymphoblastic Leukemia (ALL) accounts for majority of the childhood leukemia. Outcome of children with ALL treatment has improved dramatically. Sensitive techniques are available today for detection of minimal residual disease in children with ALL, which provide insight into the effective cytotoxic treatment. Here, we present a case study, where lymphocytes isolated from two children before and after the treatment were characterized using microscopic Fourier Transform Infrared spectroscopy. Significant changes in the absorbance and spectral pattern in the wavenumber region between 800-1800 cm-1 were found after the treatment. Preliminary analysis of the spectra revealed that the protein content decreased in the T-lymphoma patient before the treatment in comparison to the age matched controls. The chemotherapy treatment resulted in decreased nucleic acids, total carbohydrates and cholesterol contents to a remarkable extent in both B and T lymphoma patients.

Paper Details

Date Published: 27 November 2001
PDF: 8 pages
Proc. SPIE 4491, Subsurface and Surface Sensing Technologies and Applications III, (27 November 2001); doi: 10.1117/12.450167
Show Author Affiliations
Shaul Mordechai, Ben-Gurion Univ. of the Negev (Israel)
J. Mordehai, Soroka Univ. Medical Ctr. (Israel)
Jagannathan Ramesh, Ben-Gurion Univ. of the Negev (Israel)
C. Levi, Ben-Gurion Univ. of the Negev (Israel)
Mahmud Huleihal, Ben-Gurion Univ. of the Negev (Israel)
Vitaly Erukhimovitch, Ben-Gurion Univ. of the Negev (Israel)
A. Moser, Soroka Univ. Medical Ctr. (Israel)
J. Kapelushnik, Soroka Univ. Medical Ctr. (Israel)

Published in SPIE Proceedings Vol. 4491:
Subsurface and Surface Sensing Technologies and Applications III
Cam Nguyen, Editor(s)

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