Share Email Print
cover

Proceedings Paper

3D quantitative imaging of the microvasculature with the Texas Instruments Digital Micromirror Device
Author(s): Yeshaiahu Fainman; Elliott L. Botvinick; Jeffrey H. Price; David A. Gough
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

There is a growing need for developing 3D quantitative imaging tools that can operate at high speed enabling real-time visualization for the field of biology, material science, and the semiconductor industry. We will present our 3D quantitative imaging system based on a confocal microscope built with a Texas Instruments Digital Micromirror Device (DMD). By using the DMD as a spatial light modulator, confocal transverse surface (x, y) scanning can be performed in parallel at speeds faster than video rate without physical movement of the sample. The DMD allows us to programmably configure the source and the detection pinhole array in the lateral direction to achieve the best signal and to reduce the crosstalk noise. Investigations of the microcirculation were performed on 40 g to 45 g golden Syrian hamsters fit with dorsal skin fold window chambers. FITC-Dextran or Red blood cells from donor hamsters, stained with Celltracker CM-DiI, were injected into the circulation and imaged with the confocal microscope. We will present the measured results for the axial resolution, in vivo, as well as experimental results from imaging the window chamber.

Paper Details

Date Published: 8 November 2001
PDF: 8 pages
Proc. SPIE 4457, Spatial Light Modulators: Technology and Applications, (8 November 2001); doi: 10.1117/12.447746
Show Author Affiliations
Yeshaiahu Fainman, Univ. of California/San Diego (United States)
Elliott L. Botvinick, Univ. of California/San Diego (United States)
Jeffrey H. Price, Univ. of California/San Diego (United States)
David A. Gough, Univ. of California/San Diego (United States)


Published in SPIE Proceedings Vol. 4457:
Spatial Light Modulators: Technology and Applications
Uzi Efron, Editor(s)

© SPIE. Terms of Use
Back to Top