Share Email Print
cover

Proceedings Paper

Application of fluorescence-interference microscope for monitoring of dry weight dynamics and drug distribution within living cells
Author(s): Gennady G. Levin; Theodor V. Bulygin; Eugene Kalinin; Gennady N. Vishnyakov; Irene V. Goryainova
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

The authors have proposed and tested the new type of the microscope with superimposed interference and fluorescence images of the living cell. The fluorescence image gives the information about the distribution of drugs in different parts of the cell. Interference microscopy technique allows implementing the quantitative analysis of a living transparent cell, for example, to measure dry cell weight and cell density spatial distribution. Interference microscopy makes possibility the observation cell vital activity at dynamics. Computer-aided processing of interferograms using phase shift technique, allows carrying out continuous (over 12 hours and more) monitoring of living cells quantitative parameters with time interval between measurements less than one minute. RMS error for dry weight of non-living fixed test object using this microscope is about 1%.

Paper Details

Date Published: 2 November 2001
PDF: 6 pages
Proc. SPIE 4431, Photon Migration, Optical Coherence Tomography, and Microscopy, (2 November 2001); doi: 10.1117/12.447441
Show Author Affiliations
Gennady G. Levin, All-Russian Research Institute for Optophysical Measurements (Russia)
Theodor V. Bulygin, All-Russian Research Institute for Optophysical Measurements (Russia)
Eugene Kalinin, All-Russian Research Institute for Optophysical Measurements (Russia)
Gennady N. Vishnyakov, All-Russian Research Institute for Optophysical Measurements (Russia)
Irene V. Goryainova, All-Russian Research Institute for Optophysical Measurements (Russia)


Published in SPIE Proceedings Vol. 4431:
Photon Migration, Optical Coherence Tomography, and Microscopy
Stefan Andersson-Engels; Michael F. Kaschke, Editor(s)

© SPIE. Terms of Use
Back to Top