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Proceedings Paper

Characterization of the diffusion of fluorophores within microbial biofilms by flourescence correlation microscopy under two-photon excitation
Author(s): E. Guiot; M. P. Fontaine-Aupart; R. Briandet; M. N. Bellon-Fontaine; P. Georges; A. Brun; F. Merola; B. Arrio
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Paper Abstract

The penetration and diffusion capabilities of fluorophores inside microbial biofilms of heterogeneous three-dimensional structure were studied by fluorescence correlation microscopy under two-photon excitation. The influence of the size and the surface charge of the fluorophores were investigated in order to understand the mechanisms involved in molecular diffusion inhibition occuring in biofilms such as electrostatic and steric interactions.

Paper Details

Date Published: 2 November 2001
PDF: 4 pages
Proc. SPIE 4431, Photon Migration, Optical Coherence Tomography, and Microscopy, (2 November 2001); doi: 10.1117/12.447419
Show Author Affiliations
E. Guiot, Lab. Charles Fabry de l'Institut d'Optique (France)
M. P. Fontaine-Aupart, Lab. de Photophysique Moleculaire (France)
R. Briandet, Unit de Recherche en Bioadhesion et Hygiene des Materiaux (France)
M. N. Bellon-Fontaine, Unit de Recherche en Bioadhesion et Hygiene des Materiaux (France)
P. Georges, Lab. Charles Fabry de l'Institut d'Optique (France)
A. Brun, Lab. Charles Fabry de l'Institut d'Optique (France)
F. Merola, Lab. de Chimie-Physique (France)
B. Arrio, Lab. de Photophysique Moleculaire (France)


Published in SPIE Proceedings Vol. 4431:
Photon Migration, Optical Coherence Tomography, and Microscopy
Stefan Andersson-Engels; Michael F. Kaschke, Editor(s)

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