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Proceedings Paper

Application of the stretched exponential function to fluorescence lifetime imaging of biological tissue
Author(s): Jan Siegel; K.C. Benny Lee; Stephen E. D. Webb; Sandrine Leveque-Fort; Mary J. Cole; Richard Jones; Keith Dowling; Paul M. W. French; M. John Lever
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Paper Abstract

The fluorescence decay in fluorescence lifetime imaging (FLIM) is typically fitted to a multi-exponential model with discrete lifetimes. The interaction between fluorophores in heterogeneous samples (e.g. biological tissue) can, however, produce complex decay characteristics that do not correspond to such models. Although they appear to provide a better fit to fluorescence decay data than the assumption of a mono-exponential decay, the assumption of multiple discrete components is essentially arbitrary and often erroneous. The stretched exponential function (StrEF) describes fluorescence decay profiles using a continuous lifetime distribution as has been reported for tryptophan, being one of the main fluorophores in tissue. We have demonstrated that this model represents our time-domain FLIM data better than multi-exponential discrete decay components, yielding excellent contrast in tissue discrimination without compromising the goodness of fit, and it significantly decreases the required processing time. In addition, the stretched exponential decay model can provide a direct measure of the sample heterogeneity and the resulting heterogeneity map can reveal subtle tissue differences that other models fail to show.

Paper Details

Date Published: 2 November 2001
PDF: 9 pages
Proc. SPIE 4431, Photon Migration, Optical Coherence Tomography, and Microscopy, (2 November 2001); doi: 10.1117/12.447407
Show Author Affiliations
Jan Siegel, Imperial College of Science, Technology and Medicine (United Kingdom)
K.C. Benny Lee, Imperial College of Science, Technology and Medicine (United Kingdom)
Stephen E. D. Webb, Imperial College of Science, Technology and Medicine (United Kingdom)
Sandrine Leveque-Fort, Imperial College of Science, Technology and Medicine (United Kingdom)
Mary J. Cole, Imperial College of Science, Technology and Medicine (Germany)
Richard Jones, Imperial College of Science, Technology and Medicine (United Kingdom)
Keith Dowling, Imperial College of Science, Technology and Medicine (United Kingdom)
Paul M. W. French, Imperial College of Science, Technology and Medicine (United Kingdom)
M. John Lever, Imperial College of Science, Technology and Medicine (United Kingdom)


Published in SPIE Proceedings Vol. 4431:
Photon Migration, Optical Coherence Tomography, and Microscopy
Stefan Andersson-Engels; Michael F. Kaschke, Editor(s)

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