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Proceedings Paper

Active contours applied to the shape and motion analysis of amoeba
Author(s): Christophe Zimmer; Vannary Meas-Yedid; Estelle Glory; Elisabeth Labruyere; Nancy Guillen; Jean-Christophe Olivo-Marin
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Paper Abstract

We present a method based on active contours to track and segment biological cells in large image sequences obtained by video-microscopy. This task is facilitated by good time resolution and global contrast, but important obstacles are the low contrast boundary deformations known as pseudopods, as well as cell aggregations and divisions. In order to allow better detection of local boundary deformations, we adopted the gradient vector flow (GVF) model of Xu and Prince, which is defined as the steady-state solution of a reaction-diffusion problem. We discuss an undesirable effect of boundary competition in the GVF that can lead to incorrect segmentations for grey-level images. We propose to replace the steady-state solution by a transient solution of the diffusion equation to alleviate this effect, which also allows significant gains in computation time. To enhance pseudopods over texture features, we use a binary edge map obtained from a Canny-Deriche filter followed by hysteresis thresholding. We use topological operators to efficiently detect intersections and maintain contour separation between aggregating cells. Cell divisions are automatically handled by this method. We discuss limits and possible improvements of this work.

Paper Details

Date Published: 2 November 2001
PDF: 11 pages
Proc. SPIE 4476, Vision Geometry X, (2 November 2001); doi: 10.1117/12.447275
Show Author Affiliations
Christophe Zimmer, L'Institut Pasteur (France)
Vannary Meas-Yedid, L'Institut Pasteur (France)
Estelle Glory, L'Institut Pasteur (France)
Elisabeth Labruyere, L'Institut Pasteur (France)
Nancy Guillen, L'Institut Pasteur (France)
Jean-Christophe Olivo-Marin, L'Institut Pasteur (France)

Published in SPIE Proceedings Vol. 4476:
Vision Geometry X
Longin Jan Latecki; David M. Mount; Angela Y. Wu; Robert A. Melter, Editor(s)

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