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Proceedings Paper

Separation of myoglobin and hemoglobin in isolated perfused pig heart
Author(s): Robert Rauh; Michael Hiller; Tobias Trinks; Manfred D. Kessler
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Paper Abstract

For the quantitative determination of hemoglobin concentration in heart muscle it is important to distinguish between myoglobin and hemoglobin, two dyes with very similar optical absorption properties. With an isolated perfused pig heart model and EMPHO II SSK we measured tissue spectra in the visible range before and after adding erythrocytes to the perfusate. By calculating light intensity differences we were able to show spatial hemoglobin distribution in heart muscle.

Paper Details

Date Published: 31 October 2001
PDF: 5 pages
Proc. SPIE 4432, Diagnostic Optical Spectroscopy in Biomedicine, (31 October 2001); doi: 10.1117/12.447147
Show Author Affiliations
Robert Rauh, Friedrich-Alexander Univ. Erlangen-Nuernberg (Germany)
Michael Hiller, Friedrich-Alexander Univ. Erlangen-Nuernberg (Germany)
Tobias Trinks, Friedrich-Alexander Univ. Erlangen-Nuernberg (Germany)
Manfred D. Kessler, Friedrich-Alexander Univ. Erlangen-Nuernberg (Germany)

Published in SPIE Proceedings Vol. 4432:
Diagnostic Optical Spectroscopy in Biomedicine
Theodore G. Papazoglou; Georges A. Wagnieres, Editor(s)

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