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Proceedings Paper

Time-resolved autofluorescence spectroscopy of the bronchial mucosa for the detection of early cancer: clinical results
Author(s): Thomas M. Glanzmann; Pascal Uehlinger; Jean-Pierre Ballini; Alexandre Radu; Tanja Gabrecht; Philippe Monnier; Hubert van den Bergh; Georges A. Wagnieres
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Paper Abstract

Time-resolved measurements of endogenous tissue autofluorescence were carried out on the bronchial mucosa of 18 patients during endoscopy by the means of a optical fibre-based spectrometer. The objective was to assess the fluorescence lifetime as a new contrast parameter between normal and malignant tissue and to explain the origin of a previously observed contrast in fluorescence intensity. The intra- and interpatient variation of tissue autofluorescence intensity and decay on normal tissue was determined with the outcome that a strong fluctuation in autofluorescence intensity but not in lifetime was observed on the normal tissue. Preliminary results were obtained by comparing fluorescence decays on normal mucosa and dysplasia/carcinoma in situ. No significant change in fluorescence decay nor in spectrum between 510 and 650 nm was found. Measurements in parallel with an endoscopic autofluorescence imaging device, on the other hand, indicated a contrast in intensity and spectrum on the same lesions. This suggests that the spectral contrast might be due to an enhanced blood concentration in deeper lying layers of the lesion the optical fibre-based contact measurements are less sensitive to. The difference in intensity might be due to a lower concentration in fluorophores or to the thickening of the epithelium in the neoplastic mucous membrane. However, no indication for fluorescence quenching in the upper layers of the mucous membrane as the reason for the reduced fluorescence intensity was found. The fluorescence decays showed a quite stable behaviour with three decay times of 6.9 ns, 2.0 ns and 0.2 ns in the spectral range between 430 and 680 nm. This can be an indication that there is one dominant fluorophore involved, the calculated decay times suggest that it might be elastin. However, a slight spectral dependence of the fluorescence decays let presume that there is a contribution from other fluorophores, probably flavins and NADH.

Paper Details

Date Published: 31 October 2001
PDF: 11 pages
Proc. SPIE 4432, Diagnostic Optical Spectroscopy in Biomedicine, (31 October 2001); doi: 10.1117/12.447136
Show Author Affiliations
Thomas M. Glanzmann, Swiss Federal Institute of Technology (Switzerland)
Pascal Uehlinger, Swiss Federal Institute of Technology (Switzerland)
Jean-Pierre Ballini, Swiss Federal Institute of Technology (Switzerland)
Alexandre Radu, Hospices Cantonaux (Switzerland)
Tanja Gabrecht, Swiss Federal Institute of Technology (Switzerland)
Philippe Monnier, Hospices Cantonaux (Switzerland)
Hubert van den Bergh, Swiss Federal Institute of Technology (Switzerland)
Georges A. Wagnieres, Swiss Federal Institute of Technology (Switzerland)

Published in SPIE Proceedings Vol. 4432:
Diagnostic Optical Spectroscopy in Biomedicine
Theodore G. Papazoglou; Georges A. Wagnieres, Editor(s)

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