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Proceedings Paper

Fourier interferometry with spectral fluorescence imaging for the study of mitochondrial distribution and function in living normal/malignant cells
Author(s): Joseph G. Hirschberg; Elli Kohen; Cahide Kohen; Ceren Oernek; John P. Berry
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Paper Abstract

The advantages of measuring both the excitation spectrum and emission spectrum of living cells' fluorescence are reviewed together with interferometric methods. Mitochondria in yeast cells are described as examples of Biomedical methods, also including mammalian cells. Mitochondria in human hepatic cells, murine mast cells and human keratinocytes are shown using the vital stains DASPMI, Mitotracker Green and TMRE. An example in Biotechnology is the study of photosynthetic algae, used in the production of hydrogen.

Paper Details

Date Published: 24 October 2001
PDF: 18 pages
Proc. SPIE 4434, Hybrid and Novel Imaging and New Optical Instrumentation for Biomedical Applications, (24 October 2001); doi: 10.1117/12.446674
Show Author Affiliations
Joseph G. Hirschberg, Univ. of Miami (United States)
Elli Kohen, Univ. of Miami (United States)
Cahide Kohen, Univ. of Miami (United States)
Ceren Oernek, Univ. of Miami (United States)
John P. Berry, Univ. of Miami (United States)

Published in SPIE Proceedings Vol. 4434:
Hybrid and Novel Imaging and New Optical Instrumentation for Biomedical Applications
Albert-Claude Boccara; Alexander A. Oraevsky, Editor(s)

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