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Proceedings Paper

Measurements of optical tweezers stiffness
Author(s): Nunzia Malagnino; G. Pesce; Antonio Sasso; Ennio Arimondo
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Paper Abstract

We report an experimental study of the trapping efficiency of an optical tweezers system acting on spherical polystyrene particles in water solution. As force calibration method we used the viscous drag exerted by fluid flow. A parametric study of the efficiency of the trap was made as a function of bead diameter and objective NA. Optical tweezers were also characterized in terms of the optical potential well by measuring the displacement of trapped particles undergone to a viscous drag for fluid flow below the critical velocity. Trap stiffness was determined for particle size of 14.9 micrometers at different laser power.

Paper Details

Date Published: 24 October 2001
PDF: 8 pages
Proc. SPIE 4434, Hybrid and Novel Imaging and New Optical Instrumentation for Biomedical Applications, (24 October 2001); doi: 10.1117/12.446672
Show Author Affiliations
Nunzia Malagnino, INFM and Univ. degli Studi di Napoli Federico II (Italy)
G. Pesce, INFM and Univ. degli Studi di Napoli Federico II (Italy)
Antonio Sasso, INFM and Univ. degli di Napoli Federico II (Italy)
Ennio Arimondo, INFM and Univ. degli Studi di Pisa (Italy)


Published in SPIE Proceedings Vol. 4434:
Hybrid and Novel Imaging and New Optical Instrumentation for Biomedical Applications
Albert-Claude Boccara; Alexander A. Oraevsky, Editor(s)

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