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Proceedings Paper

DNA biochips based on surface-enhanced fluorescence (SEF) for high-throughput interaction studies
Author(s): Norbert Stich; Christian Mayer; Georg D. Bauer; Thomas G. M. Schalkhammer
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Paper Abstract

Molecular interaction especially biorecognitive binding can be visualized by metal cluster enhanced fluorescence. Fluorescent molecules that are bound within the electromagnetic field of a layer of metal clusters exhibit a strong boost in excitation as well as emission. We present a study, using novel surface enhanced chips in glass-slide-format, their set up, the micro arraying onto the surface, and the hybridization of oligonucleotides on these chips. Compared to standard (glass slide) DNA chips, performance, fluorescent signals as well as signal to noise ratio were considerably higher.

Paper Details

Date Published: 24 October 2001
PDF: 10 pages
Proc. SPIE 4434, Hybrid and Novel Imaging and New Optical Instrumentation for Biomedical Applications, (24 October 2001); doi: 10.1117/12.446669
Show Author Affiliations
Norbert Stich, Delft Univ. of Technology (Netherlands)
Christian Mayer, Delft Univ. of Technology (Netherlands)
Georg D. Bauer, Delft Univ. of Technology (Germany)
Thomas G. M. Schalkhammer, Delft Univ. of Technology (Netherlands)


Published in SPIE Proceedings Vol. 4434:
Hybrid and Novel Imaging and New Optical Instrumentation for Biomedical Applications
Albert-Claude Boccara; Alexander A. Oraevsky, Editor(s)

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