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Proceedings Paper

Analyzing biomolecular interactions by variable angle ellipsometry
Author(s): Jiun-Yan Wu; Chih-Kung Lee; J. H. Lee; Shuen-Chen Shiue; Shu-Sheng Lee; Shiming Lin
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Paper Abstract

In this paper, an innovative ellipsometer is developed and applied to metrology of the biomolecular interaction on a protein biochip. Both the theory, optical and opto-mechanical configurations of this newly developed ellipsometer and methodologies adopted in system design to improve the system performance are presented. It will be shown that by measuring the ellipsometric parameters, the corresponding concentration variation in biochemical reaction can be calculated according to stoichiometry analysis. By applying the variable angle ellipsometry to analysis of a multi-layered sample, the thickness and concentration are resolved. It is believed that the newly developed ellipsometer biosensor is able to undertake an accurate measurement on biomedical interaction.

Paper Details

Date Published: 24 October 2001
PDF: 4 pages
Proc. SPIE 4434, Hybrid and Novel Imaging and New Optical Instrumentation for Biomedical Applications, (24 October 2001); doi: 10.1117/12.446665
Show Author Affiliations
Jiun-Yan Wu, National Taiwan Univ. (Taiwan)
Chih-Kung Lee, National Taiwan Univ. (Taiwan)
J. H. Lee, Chung-Shan Institute of Science (Taiwan)
Shuen-Chen Shiue, National Taiwan Univ. (Taiwan)
Shu-Sheng Lee, National Taiwan Univ. (Taiwan)
Shiming Lin, National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 4434:
Hybrid and Novel Imaging and New Optical Instrumentation for Biomedical Applications
Albert-Claude Boccara; Alexander A. Oraevsky, Editor(s)

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