Share Email Print

Proceedings Paper

Diagnosis of oral tumors using light-induced autofluorescence spectra at multiple excitation wavelengths
Author(s): Wei Zheng; Malini Olivo; K. Chee Soo
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Fluorescence excitation-emission matrices (EEMs) were measured from normal and tumourous oral tissues in vitro using excitation wavelengths ranging from 220 to 500 nm. The prominent fluorescence excitation-emission peaks: (228 nm; 260/338/475 nm), (282 nm; 337/475/660 nm), (335 nm; 472 nm) and (465 nm, 523 nm) for normal tissues; (228 nm; 335/475/660 nm), (282 nm; 330/660 nm) and (350 nm; 460 nm) for tumour tissues, can be found in EEM maps, indicating the dominant fluorophores responsible for tissue autofluorescence are tryptophan, NADH, FAD, and porphyrin compounds in oral tissues. The optimal excitation wavelengths were found to be 280, 335, and 465 nm. The empirical discrimination algorithms based on I475/I660, and I520/I660 were developed. These algorithms yield an average sensitivity and specificity of 95% and 88%, respectively.

Paper Details

Date Published: 25 October 2001
PDF: 6 pages
Proc. SPIE 4597, Biophotonics Instrumentation and Analysis, (25 October 2001); doi: 10.1117/12.446649
Show Author Affiliations
Wei Zheng, National Cancer Ctr. (Singapore)
Malini Olivo, National Cancer Ctr. (Singapore)
K. Chee Soo, National Cancer Ctr. and Singapore General Hospital (Singapore)

Published in SPIE Proceedings Vol. 4597:
Biophotonics Instrumentation and Analysis
Arthur E. T. Chiou; Halina Podbielska; Steven L. Jacques, Editor(s)

© SPIE. Terms of Use
Back to Top