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Proceedings Paper

Fluorescence micro-imaging of living cells and biomolecules with ultrahigh NA objectives
Author(s): Susumu Terakawa; Takashi Tsuboi; Takashi Sakurai; Andreas Jeromin; Yoshihiko Wakazono; Seiji Yamamoto; Katsuyuki Abe
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Paper Abstract

Optical imaging with a high detecting power is very instrumental to dynamic observations of bio-molecular objects in water. To pioneer this field of imaging researches further, we used novel objective lenses of 1.65 and 1.45 in NA comparatively with 1.35 NA lens. The lenses of ultra high NA have a high resolving power and very useful properties for observation of high contrast images of fluorescently labeled molecules and subcellular organelles including DNA and membrane fusion proteins.

Paper Details

Date Published: 25 October 2001
PDF: 7 pages
Proc. SPIE 4597, Biophotonics Instrumentation and Analysis, (25 October 2001); doi: 10.1117/12.446647
Show Author Affiliations
Susumu Terakawa, Hamamatsu Univ. (Japan)
Takashi Tsuboi, Hamamatsu Univ. (Japan)
Takashi Sakurai, Hamamatsu Univ. (Japan)
Andreas Jeromin, Univ. of Toronto (Canada)
Yoshihiko Wakazono, Hamamatsu Univ. (Japan)
Seiji Yamamoto, Hamamatsu Univ. (Japan)
Katsuyuki Abe, Olympus Optical Co., Ltd. (Japan)

Published in SPIE Proceedings Vol. 4597:
Biophotonics Instrumentation and Analysis
Arthur E. T. Chiou; Halina Podbielska; Steven L. Jacques, Editor(s)

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