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Proceedings Paper

First experimental experience in adjuvant intraoperative photodynamic therapy (AIOPDT) in S117 sarcoma in mice
Author(s): Steffi Winkler; Ruediger L. Prosst; Thomas Haase; Chr. Flechtenmacher; J. Stern; Johannes Gahlen
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Paper Abstract

The effectiveness of photodynamic therapy (PDT) as an adjuvant treatment for diverse malignant tumors has been investigated in numerous studies. The therapeutic success and extent of side effects of PDT is mainly determined by the applied photosensitizer (PS) and laser energy. Adjuvant intraoperative photodynamic therapy (AIOPDT) using the PS mTHPC (meso-Tetrahydroxyphenylchlorin) causes selective tumor cell death when combined with laser irradiation of a PS specific wavelength (652 nm). Our study proved AIOPDT as an efficient modality to significantly increase postoperative recurrence-free survival after R1/R2 resection of a subcutaneously implanted soft tissue sarcoma in mice. We used mTHPC in a dose of (0,3 mg/kg BW) and a laser light energy of 5 Joule (irradiation time: 50 seconds). First results showed an increase of postoperative recurrence-free survival (Median: 103 days) in 5 animals treated with AIOPDT compared to a control group of 7 animals (Median: 20 days). The tissue specific accumulation of mTHPC was determined by point spectrofluorometry and showed a 2.28 higher PS-accumulation in the tumor center, tumor bed (1.5) and overlying skin (3.8) compared to muscle tissue (1.0) as reference parameter. Our first experimental data recommend AIOPDT to be an efficient adjuvant method to prolonge recurrence-free survival after tumor resection.

Paper Details

Date Published: 26 October 2001
PDF: 8 pages
Proc. SPIE 4433, Laser-Tissue Interactions, Therapeutic Applications, and Photodynamic Therapy, (26 October 2001); doi: 10.1117/12.446516
Show Author Affiliations
Steffi Winkler, Univ. of Heidelberg (Germany)
Ruediger L. Prosst, Univ. of Heidelberg (Germany)
Thomas Haase, German Cancer Research Ctr. (Germany)
Chr. Flechtenmacher, Univ. of Heidelberg (Germany)
J. Stern, St.-Josefs-Hospital (Germany)
Johannes Gahlen, Univ. of Heidelberg (Germany)

Published in SPIE Proceedings Vol. 4433:
Laser-Tissue Interactions, Therapeutic Applications, and Photodynamic Therapy
Reginald Birngruber; Hubert van den Bergh, Editor(s)

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