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Proceedings Paper

Addressable electrodes microarray for biological analysis system
Author(s): Mihaela Ilie; Elena Cianci; Vittorio Foglietti; Antonio Minotti; Gianluca de Bellis; Giancarlo Caramenti
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Paper Abstract

Multidisciplinary efforts, combining microfabrication, chemistry and molecular biology, have been recently focused on the development of large electrode arrays loaded with oligonucleotide probe to allow rapid analysis of nucleic acid samples. Different micromachining techniques can be used for obtaining the inlet, outlet and main reservoirs for the analyte. In the present work silicon wafers are used as substrates for the microarrays, patterned by means of direct writing or optical lithography. Three methods are developed in order to obtain reservoirs with depths ranging from 5 microns to 200 microns, allowing an analyte volume in the range of 1 nl to 1 ml: reactive ion etching of a polyimide layer, wet anisotropic etching of silicon, respectively deep wet isotropic etching of the glass cover. The glass cover is bonded at low temperature, using spin-on glass as adhesive and ensures a protection of the analyte, as well as a rapid entering of the analyte in the reservoirs, increasing thus the speed of the analysis. A custom laser induced fluorescence set-up is used in order to perform the analysis. The fluorescent DNA molecules are concentrated and localized during an observation time of 60 seconds, proving the functionality of the device.

Paper Details

Date Published: 28 September 2001
PDF: 8 pages
Proc. SPIE 4560, Microfluidics and BioMEMS, (28 September 2001); doi: 10.1117/12.443063
Show Author Affiliations
Mihaela Ilie, Istituto de Elettronica dello Stato Solido and Univ. Politehnica of Bucharest (Italy)
Elena Cianci, Istituto de Elettronica dello Stato Solido (Italy)
Vittorio Foglietti, Istituto de Elettronica dello Stato Solido (Italy)
Antonio Minotti, Istituto de Elettronica dello Stato Solido and Univ. di Roma III (Italy)
Gianluca de Bellis, Istituto de Technologie Biologiche Avanzate (Italy)
Giancarlo Caramenti, Istituto de Technologie Biologiche (Italy)

Published in SPIE Proceedings Vol. 4560:
Microfluidics and BioMEMS
Carlos H. Mastrangelo; Holger Becker, Editor(s)

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