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Proceedings Paper

Visualization of 3-D phase structure in confocal and conventional microscopy
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Paper Abstract

A reflection differential interference contrast (DIC) system has been investigated as a means for imaging phase information in confocal microscopy. Interestingly, this method has an advantage over the split-detector method for differential phase contrast (and its corresponding confocal derivative) in that the background signal decays as the specimen surface is displaced away from the focal plane. Therefore, a series of DIC images from successive planes of focus can be used to produce easily visualized three-dimensional reconstructions.

Paper Details

Date Published: 1 July 1991
PDF: 6 pages
Proc. SPIE 1450, Biomedical Image Processing II, (1 July 1991); doi: 10.1117/12.44306
Show Author Affiliations
Carol J. Cogswell, Univ. of Sydney (Australia)
Colin J. R. Sheppard, Univ. of Sydney (Australia)

Published in SPIE Proceedings Vol. 1450:
Biomedical Image Processing II
Alan Conrad Bovik; Vyvyan Howard, Editor(s)

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