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Proceedings Paper

Background correction in multiharmonic Fourier transform fluorescence lifetime spectroscopy
Author(s): Kerry M. Swift; George Weston Mitchell
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Paper Abstract

We report here a method for measuring and eliminating artifacts caused by background in fluorescence lifetime or rotational correlation data using multiharmonic Fourier transform frequency domain fluorometry. A single measurement on a blank yields phase and amplitude values for multiple modulation frequencies simultaneously, and these may then be used to correct the sample's data. Experimental results demonstrate the applicability of the method over a wide range of background contributions.

Paper Details

Date Published: 1 May 1991
PDF: 8 pages
Proc. SPIE 1431, Time-Resolved Spectroscopy and Imaging of Tissues, (1 May 1991); doi: 10.1117/12.44188
Show Author Affiliations
Kerry M. Swift, SLM Instruments, Inc. (United States)
George Weston Mitchell, SLM Instruments, Inc. (United States)

Published in SPIE Proceedings Vol. 1431:
Time-Resolved Spectroscopy and Imaging of Tissues
Britton Chance, Editor(s)

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