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Proceedings Paper

Quantitative analysis of hemoglobin oxygenation state of rat head by time-resolved photometry using picosecond laser pulse at 1064 nm
Author(s): Yasutomo Nomura; Mamoru Tamura
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Paper Abstract

By the use of the picosecond laser pulse of near-infrared light at 1064 nm, the temporal profile of the transmitted light through the anesthetized rat head has been investigated. The light intensity at a certain time after the input pulse was exponentially attenuated by hemoglobin in the blood, although the transmitted pulse broadened markedly due to scattering of the cerebral tissue. The optical pathlength which is required for the quantitation of the absolute absorbance change, was directly determined by the measurement of time of flight of light pulses, as the product of v and t where v is the velocity of light in water (0.23 mm/ps) and t, time in picosecond. The temporal profiles of transmitted light through the rat head were measured with changing the oxygen concentration in the inspired gas and cerebral venous oxygen saturation of hemoglobin (SvO2) were obtained quantitatively in the various conditions. The SvO2 values obtained from the time of flight measurement agreed with those of gas analysis of blood withdrawn from the internal jugular vein. Thus, the picosecond laser pulse technique is essential to quantify SvO2.

Paper Details

Date Published: 1 May 1991
PDF: 8 pages
Proc. SPIE 1431, Time-Resolved Spectroscopy and Imaging of Tissues, (1 May 1991); doi: 10.1117/12.44182
Show Author Affiliations
Yasutomo Nomura, Communications Research Lab./Ministry of Posts and Telecommu (Japan)
Mamoru Tamura, Hokkaido Univ. (Japan)

Published in SPIE Proceedings Vol. 1431:
Time-Resolved Spectroscopy and Imaging of Tissues
Britton Chance, Editor(s)

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