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Proceedings Paper

Photon correlation spectroscopic studies of filamentous actin networks
Author(s): Jay Edward Newman; Pier L. San Biagio; Kenneth L. Schick
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Paper Abstract

Actin plays a major role in the structural integrity and motility of cells as well as in the intracellular dynamics of other macromolecules. Photon Correlation Spectroscopy (PCS) has been used to monitor the diffusion of polystyrene latex spheres (PLS) of different sizes within in vitro polymerized actin solutions under a variety of conditions. Specific actin-binding proteins were added to regulate the actin filament lengths as well as to cross-link filaments together. PCS measurements give information on the mobility of PLS over probing distances equal to the inverse scattering vector magnitude which range from 40 to 420 nm for the data. Results allow estimation of the mean pore sizes within the actin networks as a function of both actin concentration (0.4 - 5 mg/ml) and the presence of actin-binding proteins. Probe diffusion coefficients were measured for PLS samples in length-regulated actin networks at a fixed actin concentration, c (0.65 mg/ml) as c*, the semi-dilute overlap concentration, was varied. There was a change in the dependence on c/c* near the value 1 at which the filaments just overlap. Quasi-static low-angle diffraction patterns from actin solutions have been detected and quantified using a CCD video camera/frame grabber system to study the spatial and slow temporal variations in the intensity patterns.

Paper Details

Date Published: 1 June 1991
PDF: 20 pages
Proc. SPIE 1430, Photon Correlation Spectroscopy: Multicomponent Systems, (1 June 1991); doi: 10.1117/12.44159
Show Author Affiliations
Jay Edward Newman, Union College (United States)
Pier L. San Biagio, Union College (United States)
Kenneth L. Schick, Union College (United States)


Published in SPIE Proceedings Vol. 1430:
Photon Correlation Spectroscopy: Multicomponent Systems
Kenneth S. Schmitz, Editor(s)

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