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Proceedings Paper

HIV detection by in-situ hybridization based on confocal reflected light microscopy
Author(s): Louis C. Smith; Zeljko Jericevic; Roland Cuellar; Stephen W. Paddock; Dorothy E. Lewis
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Paper Abstract

Elucidation of the pathogenesis of AIDS is confounded by the finding that few actively infected CD4+ cells (1 in 104-105) can be detected in the peripheral blood, even though there is dramatic depletion (often >90%) of CD4+ cells as the disease progresses. A sensitive, 35S-based human immunodeficiency virus (HIV) mRNA in situ hybridization technique was coupled with a new detection method, confocal laser scanning microscopy, to examine transcriptionally active HIV-infected cells from individuals at different disease stages. An algorithm for image segmentation and analysis has been developed to determine the proportion of HIV-positive cells. Data obtained using this improved detection method suggest that there are more HIV mRNA-producing cells in HIV-infected individuals than previously thought, based on other detection methods.

Paper Details

Date Published: 1 May 1991
PDF: 9 pages
Proc. SPIE 1428, Three-Dimensional Bioimaging Systems and Lasers in the Neurosciences, (1 May 1991); doi: 10.1117/12.44144
Show Author Affiliations
Louis C. Smith, Baylor College of Medicine (United States)
Zeljko Jericevic, Baylor College of Medicine (United States)
Roland Cuellar, Baylor College of Medicine (United States)
Stephen W. Paddock, Univ. of Wisconsin/Madison (United States)
Dorothy E. Lewis, Baylor College of Medicine (United States)


Published in SPIE Proceedings Vol. 1428:
Three-Dimensional Bioimaging Systems and Lasers in the Neurosciences
James E. Boggan; Leonard J. Cerullo; Louis C. Smith, Editor(s)

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