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Proceedings Paper

Reduced defocus degradation in a system for high-speed three-dimensional digital microscopy
Author(s): Laura A. Quesenberry; V. Arlene Morris; Ian R. Neering; Stuart R. Taylor
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Paper Abstract

Most low-light-level 3D bio-imaging systems use sensors with high spatial resolution but relatively low temporal resolution. The authors aim to measure events in skeletal muscle cells that include (1) changes in cross-sectional area during contraction and (2) regions of contracting cells illuminated by Ca2+-activated photoproteins compared with those that are dark or partly lit. This paper outlines some limits of the system's ability to detect these events.

Paper Details

Date Published: 1 May 1991
PDF: 14 pages
Proc. SPIE 1428, Three-Dimensional Bioimaging Systems and Lasers in the Neurosciences, (1 May 1991); doi: 10.1117/12.44140
Show Author Affiliations
Laura A. Quesenberry, Mayo Foundation (United States)
V. Arlene Morris, Mayo Foundation (United States)
Ian R. Neering, Mayo Foundation (United States)
Stuart R. Taylor, Mayo Foundation (United States)


Published in SPIE Proceedings Vol. 1428:
Three-Dimensional Bioimaging Systems and Lasers in the Neurosciences
James E. Boggan; Leonard J. Cerullo; Louis C. Smith, Editor(s)

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