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Further study of trichosanthin's effect on mouse embryos with confocal laser scanning microscopy
Author(s): Hui Xu; Chunyang Zhang; Hui Ma; Die Yan Chen
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Paper Abstract

Trichosanthin(TCS), a ribosome inactivating protein extracted from the root tuber of a traditional Chinese medicine herb Tian Huo Fen(THF), possessed abortifacient, anti-tumor and anti-human immunodeficiency virus(HIV) activities. For centuries in China, THF has been used as an effective folk medicine to terminate early and midtrimester pregnancies and to treat ectopic pregnancies, hydatidiform moles and trophoblastic tumor. We observed the changes in reactive oxygen species and intracellular calcium in mouse embryos induced by TCS with confocal laser scanning microscopy in combination with the fluorescene diacetate (DCFHDA) and Fluo-3-AM. The results indicated that TCS induced increase in intracellular calcium and production of reactive oxygen species in mouse embryos , and TCS inhibited the development of mouse embryos effectively. Mouse embryos of different developmental stages before implantation are used in the experiments. This provides new insight into mechanism for abortifacient activity of TCS.

Paper Details

Date Published: 25 September 2001
PDF: 3 pages
Proc. SPIE 4548, Multispectral and Hyperspectral Image Acquisition and Processing, (25 September 2001); doi: 10.1117/12.441372
Show Author Affiliations
Hui Xu, Tsinghua Univ. (China)
Chunyang Zhang, Tsinghua Univ. (China)
Hui Ma, Tsinghua Univ. (China)
Die Yan Chen, Tsinghua Univ. (China)

Published in SPIE Proceedings Vol. 4548:
Multispectral and Hyperspectral Image Acquisition and Processing

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