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Proceedings Paper

Promotional effects of CO2 laser on neoplastic lesions in hamsters
Author(s): Jeffrey S. Kingsbury; Joseph E. Margarone; S. Satchidanand; Charles Liebow
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Paper Abstract

Surgical incision may have promotional effects on neoplastic lesions, possibly through release of tissue growth factors (e.g., EGF, FGF(beta) , IGF, TGF(alpha) ). The CO2 laser may precipitate altered release of these factors. To test this, .5 cm laser, and scalpel incisions were made into fields treated by application of .5% DMBA in acetone, 3 times a week for 6 weeks (group 1) and 12 weeks (group 2). DMBA is a complete carcinogen (initiator and promoter). At 6 weeks, chemically, but not histologically, definable premalignant lesions are seen. Treatment for 12 weeks causes histologic neoplasia which can be graded with T-N-M classification. For both groups, the surgical sites were examined clinically and histologically 4 weeks post-op in a blind fashion. Standard criteria were utilized for defining neoplasia. For group 1, 3 out of 6 laser treated animals developed large exophytic squamous cell carcinomas, but no lesions developed in 12 contralateral, 3 control and 3 scalpel treated pouches. For group 2, 12 of 16 laser treated animals developed tumor with mean grade of 1.75 and mean size of 7.4 mm, 5 of 6 scalpel treated animals developed tumor with mean grade of 1.83 and mean size of 3.6 mm and 3 of 6 control animals developed tumor with mean grade of 1.00 and mean size of 1.5 mm. By the Student 't' test on the binomial distribution lasers cause significant promotion (p < .01). These results suggest that laser surgery may have earlier and more profound promotional effects than scalpel on initiated cells relative to tumor size in the vicinity of the wound site by increased release of growth factors.

Paper Details

Date Published: 1 June 1991
PDF: 5 pages
Proc. SPIE 1427, Laser-Tissue Interaction II, (1 June 1991); doi: 10.1117/12.44122
Show Author Affiliations
Jeffrey S. Kingsbury, SUNY/Buffalo School of Dental Medicine and Buffalo General Hospital (United States)
Joseph E. Margarone, SUNY/Buffalo School of Dental Medicine and Buffalo General Hospital (United States)
S. Satchidanand, Buffalo General Hospital (United States)
Charles Liebow, SUNY/Buffalo School of Dental Medicine and Buffalo General Hospital (United States)


Published in SPIE Proceedings Vol. 1427:
Laser-Tissue Interaction II
Steven L. Jacques, Editor(s)

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