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Proceedings Paper

Measurement of light scattering from cells using an inverted infrared optical trap
Author(s): William H. Wright; Gregory J. Sonek; Yasuyuki Numajiri; Michael W. Berns
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Paper Abstract

A new inverted laser trapping microscope, combined with a linear diode array, has been developed to measure light scattering from a single cell over a large (up to approximately equals 45 degree(s)) angular range in real time. Diffraction profiles from 5 and 10 micrometers diameter polystyrene dielectric microspheres are in qualitative agreement with Lorenz-Mie calculations in terms of the separation and number of side-lobe features. Diffraction profiles from red blood cells (RBC), Chinese Hamster Ovary (CHO) cells, and liposomes exhibit features that can ultimately be correlated to their size and shape. Results obtained with this instrument can be used to determine the optical properties of the trapped cell, the location of the cell in the optical trap with respect to the laser focal point, and the forces acting on the cell.

Paper Details

Date Published: 1 June 1991
PDF: 9 pages
Proc. SPIE 1427, Laser-Tissue Interaction II, (1 June 1991); doi: 10.1117/12.44113
Show Author Affiliations
William H. Wright, Beckman Laser Institute and Medical Clinic/Univ. of California, Irvine (United States)
Gregory J. Sonek, Univ. of California/Irvine (United States)
Yasuyuki Numajiri, Beckman Laser Institute and Medical Clinic/Univ. of California, Irvine (United States)
Michael W. Berns, Beckman Laser Institute and Medical Clinic/Univ. of California, Irvine (United States)


Published in SPIE Proceedings Vol. 1427:
Laser-Tissue Interaction II
Steven L. Jacques, Editor(s)

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