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Proceedings Paper

Intraoperative metastases detection by laser-induced fluorescence spectroscopy
Author(s): Sandor G. Vari; Theodore G. Papazoglou; Maurits J. van der Veen; Michael C. Fishbein; J. D. Young; Mudjianto Chandra; Thanassis Papaioannou; Clain Beeder; Wei-Qiang Shi; Warren S. Grundfest
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Paper Abstract

The authors studied the ability of Laser Induced Fluorescence Spectroscopy (LIFS) for the intraoperative identification of metastases using a photosensitizing agent Photofrin IIr to enhance spectroscopic detection. A He-Cd laser source (442 nm) was used to produce low-power illumination of tissue via a hand-held 400 micrometers fiberoptic probe. Through the same fiber, reflected and emitted light was returned to an optical multi-channel analyzer (OMA III) for analysis. Spectroscopic signals were displayed on a screen for immediate examination. Lobund Wistar rats, inoculated with Pollard rat adenocarcinoma cells, were used as an animal model. Photofrin IIr was administered intraperitoneal 24 or 48 hours prior to surgical exploration in doses varying from 0.75-7.5 mg/kg. Metastases detection was performed during abdominal exploration directed to ipsilateral and contralateral inguinal, iliac, para-aortic and renal lymph nodes. Nineteen tissue samples, identified as abnormal by LIFS, were removed for histologic analysis; 11 of these samples were larger than 5mm and histologic examination revealed malignancy in all cases. While LIFS signals showed malignancy in 8 tissue samples with dimensions less than 5mm, histology confirmed this in only 3. However, serial histologic sections were not performed. From the initial results, it was concluded that LIFS detection of malignant tissue is feasible and enhanced by the addition of Photofrin IIr. LIFS may be a promising technique for the intraoperative detection of primary malignant and metastatic tissue.

Paper Details

Date Published: 1 June 1991
PDF: 10 pages
Proc. SPIE 1426, Optical Methods for Tumor Treatment and Early Diagnosis: Mechanisms and Techniques, (1 June 1991); doi: 10.1117/12.44051
Show Author Affiliations
Sandor G. Vari, Cedars-Sinai Medical Ctr. (United States)
Theodore G. Papazoglou, Cedars-Sinai Medical Ctr. (United States)
Maurits J. van der Veen, State Univ. Utrecht School of Medicine (Netherlands)
Michael C. Fishbein, Cedars-Sinai Medical Ctr. (United States)
J. D. Young, Cedars-Sinai Medical Ctr. (United States)
Mudjianto Chandra, Cedars-Sinai Medical Ctr. (United States)
Thanassis Papaioannou, Cedars-Sinai Medical Ctr. (United States)
Clain Beeder, Cedars-Sinai Medical Ctr. (United States)
Wei-Qiang Shi, Cedars-Sinai Medical Ctr. (United States)
Warren S. Grundfest, Cedars-Sinai Medical Ctr. (United States)

Published in SPIE Proceedings Vol. 1426:
Optical Methods for Tumor Treatment and Early Diagnosis: Mechanisms and Techniques
Thomas J. Dougherty, Editor(s)

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