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Proceedings Paper

Fiber optic biosensor using aptamer as receptors
Author(s): Shuqin Yu; Xiaokun Cai; Xianglin Tan; Yexiang Zhu; Bin Lu
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Paper Abstract

Reagentless biosensor that can directly transducer molecular recognition to optical signal should potentiate the development of sensor array fora wide variety of analytes. Nucleic acid aptamer can bind ligand tightly and specifically with conformational change of aptamer, and can be used as a receptor in biosensor. We have therefore developed a fiber-optic biosensor by aptamer connected with molecular beacon. Molecular beacons consist of an oligonucleotide sequence containing complementary sequence sections at either end. These two sequence containing segments base pair with each other to form a hairpin shaped loop structure, the fluorophore and quencher were attached at 5 foot- and 3 foot-end of molecular beacon respectively. When thrombin binding to the stem-loop of molecular beacon aptamer, the pseudoknot structure was interrupted, resulting a release of fluorescence from quenching and a increase in fluorescence emission. This novel biosensor system in this project has a large potential and is specific and sensitivity. A similar strategy could be used to study other analytes such as protein and small molecules.

Paper Details

Date Published: 14 September 2001
PDF: 3 pages
Proc. SPIE 4414, International Conference on Sensor Technology (ISTC 2001), (14 September 2001); doi: 10.1117/12.440213
Show Author Affiliations
Shuqin Yu, Huazhong Univ. of Science and Technology (China)
Xiaokun Cai, Huazhong Univ. of Science and Technology (China)
Xianglin Tan, Huazhong Univ. of Science and Technology (China)
Yexiang Zhu, Huazhong Univ. of Science and Technology (China)
Bin Lu, Huazhong Univ. of Science and Technology (China)

Published in SPIE Proceedings Vol. 4414:
International Conference on Sensor Technology (ISTC 2001)
Yikai Zhou; Shunqing Xu, Editor(s)

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