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Proceedings Paper

Direct electrochemistry of pentachlorophenol hydroxylase
Author(s): Wei-hong Xie; H. Allen; O. Hill; Luet L. Wong
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Paper Abstract

The direct electrochemistry of flavin-containing monooxygenase, pentachlorophenol hydroxlase (PCPH), has been investigated under a variety of conditions. PCPH underwent a two-electron process on the electrodes, which correspond to the reduction/oxidation of FAD/FADH2 within the enzyme. The electrochemical response of PCPH relies on the interface of the electrode and the enzyme solution. Three types of interaction of the enzyme molecule with the electrodes were observed: at a bare edge-plane graphite electrode, diffusion-controlled process was observed, suggesting the interaction is weak. In the presence of the cations, the interaction became stronger, so that the voltammetric response changed from a diffusion control to an adsorption control An intermediate case was observed at a poly(L- lysine) modified EPG electrode.

Paper Details

Date Published: 14 September 2001
PDF: 4 pages
Proc. SPIE 4414, International Conference on Sensor Technology (ISTC 2001), (14 September 2001); doi: 10.1117/12.440184
Show Author Affiliations
Wei-hong Xie, Wuhan Institute of Virology (China)
H. Allen, Univ. of Oxford (United Kingdom)
O. Hill, Univ. of Oxford (United Kingdom)
Luet L. Wong, Univ. of Oxford (United Kingdom)


Published in SPIE Proceedings Vol. 4414:
International Conference on Sensor Technology (ISTC 2001)

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