Share Email Print
cover

Proceedings Paper

Molecular recognition technology for detection of protein target by molecular beacon
Author(s): Shunqing Xu; Xiaokun Cai; Xianglin Tan; Bin Lu
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Until recently, molecular beacons were limited to detecting nucleic acid target. Here we present a new technology for detection of thrombin protein by aptamer-derived molecule beacon without any antibody. A aptamer anti-thrombin was used as a recognition molecule. To make aptamer molecular beacon, we constructed two DNA oligomers that are derived from the aptamer to thrombin. To one of the DNA oligomers that forms a hairpin structure, the fluorophore and quencher were attac3hed at the 5 foot and 3 foot ends, respectively. Specially in the presence of Tat or its peptides, but not in the presence of other proteins, the two ligometers undergo a conformational change to form a duplex that leads to relieving of fluorophore from the quencher, and thus a significant enhancement of the fluorescence of fluorescein was observed. A novel strategy for exploiting aptamers in the analysis of thrombin has been described. A similar strategy could be used to study other analytes such as proteins and small molecules.

Paper Details

Date Published: 14 September 2001
PDF: 3 pages
Proc. SPIE 4414, International Conference on Sensor Technology (ISTC 2001), (14 September 2001); doi: 10.1117/12.440170
Show Author Affiliations
Shunqing Xu, Huazhong Univ. of Science and Technology (China)
Xiaokun Cai, Huazhong Univ. of Science and Technology (China)
Xianglin Tan, Huazhong Univ. of Science and Technology (China)
Bin Lu, Huazhong Univ. of Science and Technology (China)


Published in SPIE Proceedings Vol. 4414:
International Conference on Sensor Technology (ISTC 2001)

© SPIE. Terms of Use
Back to Top